Jefferson Fernando Naves Pinto
Abstract:
Aimed to characterize the genetic variability of guariroba from four regions of the Goiás state through morphological and molecular descriptors, with the purpose of providing information about the genetic diversity of the species and its use in future breeding work. In the morphological analysis, 18 descriptors were evaluated in 36 provenances from guariroba from four regions of Goiás State. Molecular characterization was performed using RAPD (Random Amplified Polimorfic DNA) molecular markers generated by 16 decamer primers in 8 guariroba accessions from the four study regions. Morphological data were submitted to univariate and multivariate statistical analysis, and descriptors with eigenvalues lower than 0.70 were discarded. The molecular markers obtained were converted into a binary data matrix, from which the genetic distances between accessions were estimated. After morphological and molecular analysis, the cluster analysis and graphic dispersion were performed. It was found that 55% of the descriptors analyzed are unnecessary for the morphological characterization of guariroba. This discard did not cause loss of information. In the grouping of provenances, there was no evidence of grouping by region of collection, indicating that even though they are geographically close, they are genetically divergent. Based on molecular data, a total of 116 markers were obtained, of which 20.69% were monomorphic. Genetic distances between accessions ranged from 0.27 to 0.48. These molecular markers demonstrated the high genetic variability of ix accessions, which were not grouped by collection region. Based on all the analyzes, it's concluded that isn't necessary to collect guariroba accessions in a large number of regions. Collection in nearby regions is sufficient, since there is good representation of genetic variability between accessions collected in geographically close regions.
