Kerlley Cristina de Assis
Abstract:
Anacardium othonianum Rizz., known as cerrado cashew tree, is a fruit and medicinal species native to this biome with great economic potential. Since agricultural expansion in the Cerrado region has been accompanied by a reduction in native vegetation, micropropagation has significantly contributed to the spread of various species, leading to uniform sanitary seedlings for commercial planting and recovery of degraded areas. In this work techniques were applied to study some factors that interfere with micropropagation of A. othonianum Rizz. There are no reports in the literature about studies of such aspects for this species. The results indicate that the seed storage condition was satisfactory in the supply of explants for use in micropropagation assays for up to 275 days. With the use of nodal segments it was observed that MS (50 and 25%) and WPM (100 and 50%) media were more efficient in seedling regeneration and the best medium volumes were 15 and 25 mL in assay containing MS medium (50%). Culture media supplemented with up to 8.88 µM BAP and 4.44 µM ANA had no effect on nodal segment multiplication. Furthermore, it was found that the best morphophysiological response was obtained by inoculating the nodal segments in horizontal orientation in the presence of light, and that sucrose and cotton plug influence the adaptation of A. othonianum Rizz seedlings produced in vitro.
