Cristiane Iracema Monteiro Estevão
Abstract:
Due to the inadequate use of native areas and species of the Cerrado biome, the risk of extinction and loss of genetic diversity is becoming increasingly evident and worrying the scientific community. Studies referring to genetic characterization based on morphological and molecular descriptors can support information that enable the implementation of species breeding and conservation strategies. The present study aimed to genetically characterize 93 genotypes of Campomanesia spp. from the Goiana Germplasm Collection of Campomanesia spp from UFG - Jataí Regional, based on 17 morphological descriptors and 13 ISSR primers. The 93 genotypes were subdivided into three groups: flowering in 2013 (F13); flowering in 2014 (F14); and no flowering during the evaluation periods (NF). A high coefficient of variation was found for the morphological descriptors demonstrating the existence of genetic variability in the 93 genotypes of Campomanesia spp. In these cases, the existence of variation indicates the possibility of using the germplasm collection to obtain gains in the improvement of the gabirobeira. The morphological descriptors: plant height in the reproductive stage in the third evaluation (AP R3) and leaf width in the reproductive stage in the second evaluation (LF V2), contributed the most to the total variance of genotypes F13 and F14. ISSR primers amplified 105 bands with 100% polymorphism demonstrating the existence of genetic variability in the studied genotypes. Molecular analysis of variance (AMOVA) showed that most of the variation is contained within the groups (genotypes that flowered in 2013 F13, 2014 F14 and not flowered NF) representing 97.82% of total variability. This indicates that pre-breeding strategies should focus on analysis within groups.
