Lara Rodrigues de Queiroz Carvalho
Abstract:
The aim of this study was to evaluate the effect of two fermentation periods together with bacterial inoculant and/or fibrolytic enzyme on the nutritional value of sugarcane silage. Sugarcane was packed in mini-silo with capacity of 5L and average density of 830 kg/m³. The experimental design was completely randomized with three replications, in a 2 x 3 x 2 factorial scheme. Two fermentation periods (30 and 100 d), three doses of cellulase enzyme (0, 3 and 6%) and inoculant application or not bacterial (Lactobacillus plantarum + Pediococcus pentosaceus, 2.5 x 1010 CFU/g). Bromatological composition, total digestible nutrients (NDT), dry matter (MS) and neutral detergent fiber (FDN) degradability and stability rate were evaluated. Bacterial inoculant and cellulase enzyme did not significantly affect pH, organic matter (MO) and hemicellulose, however, these variables were affected by the fermentation period where MS decreased (P <0.05) from 22 to 17. %, MO from 96% to 95% and hemicellulase from 25.8 to 20.9% and the pH increased from 3.3 to 3.4. Titratable acidity and FDN and FDA contents had a triple interaction between the studied factors. Treatment with 100 days of fermentation without inoculant and 6% cellulase had the best response in titratable acidity (21.6 ml). The FDN, FDA and NDT contents were higher in the longest fermentation period (100 days) with the addition of 6% cellulase without bacterial inoculant (44.9, 27.2 and 64.0% for FDN, FDA and NDT respectively). The highest potential degradability of MS was at 100 days of fermentation. The 3% concentration of cellulase associated with the inoculant increased the potential degradability of MS from 65.9 to 73.1% at 100 days of fermentation. There was greater effective MS degradability and FDN degradability decrease in cellulase addition treatments. The inoculant favored the aerobic stability rate. There was a double interaction of cellulase x period in which the highest stability was at 30 days without cellulase addition. The addition of a bacterial inoculant or cellulase enzyme was efficient in improving the nutritional value of sugarcane silage, with better results when used independently.
