Valéria Prado Braga
Abstract:
The gabirobeira (Campomanesia adamantium) is a Cerrado fruit species, its fruit has commercial potential in fresh consumption and in the food industry. However, gabiroba seeds have recalcitrant behavior. Species with this behavior can be conserved by plant tissue culture techniques such as synthetic seeds. Therefore, the aim of this work was to establish the protocol for the production of synthetic seeds of Campomanesia adamantium from true seeds. For seed encapsulation, 4 sodium alginate concentrations (0.1.5, 2 and 3%) and 3 complexation times (15, 20 and 30 minutes) were tested, with no significant interaction between the studied factors, however. As alginate concentration increased, germination time increased and germination capacity decreased. Synthetic seeds with the same alginate concentrations were produced within 20 minutes of complexation and stored at 4ºC for 1, 7, 15, 30 and 60 days in order to evaluate their storage capacity. The concentrations of 2% sodium alginate managed to preserve the integrity of encapsulated seed membranes regardless of storage time, however this wasn't enough to promote germination.
